M. Ahmadinasab; S. Valipour Chahardah Cheric; M. Setorki
Abstract
Diabetes mellitus is associated with physical, physiological, and pathological changes in the liver tissue. The aim of this study was to investigate the protective effects of hydroalcoholic extract of Crataegus monogyna Jacq. on histopathology and liver function in streptozotocin (STZ)-induced diabetic ...
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Diabetes mellitus is associated with physical, physiological, and pathological changes in the liver tissue. The aim of this study was to investigate the protective effects of hydroalcoholic extract of Crataegus monogyna Jacq. on histopathology and liver function in streptozotocin (STZ)-induced diabetic rats. In this experimental study, Wistar male rats were randomly divided into six groups of eight including control, diabetic, diabetic treated with 100, 200, and 400 (mg/kg) doses of C. monogyna extract and positive control (diabetic treated with captopril). Diabetes was induced by intra-peritoneal injection of a single-dose of STZ (60 mg/kg). After observation of the diabetes symptoms (blood glucose level above 250 mg/dL and polydipsia), treatments were done using extract and drug. After three weeks of treatment with extract and drug intra-peritoneally, the serum level of liver enzymes and albumin was determined and liver tissue samples were prepared for histopathologic examination. In diabetic rats, there was a significant decrease in serum albumin and a significant increase in serum glutamic-pyruvic transaminase (GPT) and gamma glutamyltransferase (GGT) in comparison with healthy rats (P<0.05). Treatment of diabetic rats with different doses of C. monogyna extract increased albumin and decreased GGT in serum significantly (P<0.05). In diabetic rats, hepatocytes necrosis, complete destruction of the liver lobules and Remak bundles and hyperemia were observed, and the treatment with C. monogyna extract prevented the pathologic changes. The results of this study showed that C. monogyna extract can prevent liver tissue degradation and its dysfunction in diabetic rats.
Sh. Tajmirali; Mahbubeh Setorki; Z. Hooshmandi
Abstract
Given the role of inflammation and pain, causing delayed diseases recovery, as well as the side effects of chemical drugs and traditional uses of Satureja bachtiarica Bunge, this study was aimed to evaluate the analgesic and anti-inflammatory effects of S. bachtiarica essential oil. In this experimental ...
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Given the role of inflammation and pain, causing delayed diseases recovery, as well as the side effects of chemical drugs and traditional uses of Satureja bachtiarica Bunge, this study was aimed to evaluate the analgesic and anti-inflammatory effects of S. bachtiarica essential oil. In this experimental study, analgesic and anti-inflammatory effects of S. bachtiarica essential oil were evaluated using acetic acid and xylene tests. In each test, male NMRI mice were randomly divided into five groups including control (normal saline, IP), positive control (indomethacin at a dose of 10 mg/kg/dexamethasone at a dose of 2mg/kg, IP) and intervention groups (Satureja bachtiarica essential oil at doses of 50, 75 and 100 mg/kg, IP). The collected data were analyzed using SPSS software. According to the results, S. bachtiarica essential oil at doses of 25, 50 and 100 mg/kg and indomethacin caused significant analgesic effects compared to the control group (P<0.001), and higher analgesic effect was recorded for the dose of 100mg/kg showed. In xylene test, inflammation in groups receiving S. bachtiarica essential oil at doses of 25, 50 and 100 mg/kg and dexamethasone was significantly lower than that of control group (P<0.001). Our results clearly showed the positive effects of Satureja bachtiarica essential oil in reducing inflammation and pain.